dr tim jackson glutathione Research Day 2026 Guest Speaker - Surgery Dr. Professor Timothy Jackson -

After the GST-tag was cleaved by PreScission protease (GE Healthcare), the proteins were further purified by a second glutathione Sephararose 4B column, the collected fractions containing target proteins were then loaded into MonoQ column for further purification with a linear gradient elution, and fractions containing VanR or mutant VanR from MonoQ were finally purified through Supperdex 75 (GE Healthcare) column equilibrated with buffer (20 mM Tris, pH 8.0, 200 mM NaCl, 2 mM DTT)

In view of robust patient blood management practices and despite stringent selection criteria, only ~ 56% of enrolled subjects were transfused (site range 1882%)

My boss at the time was Dr

It was found that among the identified genes, Kat5, Tp53, Rela, Hif1a, Hspe1, Psma1, Sat1, Eif3d, Gnb2l1, and Eef1g were the top 10 hub genes (Figure 6B)