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FIGURE 1 In the original analysis, classification appeared to be supported by functional data such as substrate selectivity and inhibition characteristics ( Discovery of Ketosteroid Isomerase In 1955 Talalay and Wang discovered a new enzymatic reaction involving ketosteroids, including 5-androsten-3,17-dione (5-AD) and 5-pregnen-3,20-dione (5-PD), and purified a highly active isomerase from Pseudomonas bacteria ( The enzyme, ketosteroid isomerase, obtained from several bacterial species has subsequently been extensively investigated by incisive structural and functional studies owing to its extraordinary catalytic efficiency (Figure 2)

The PPP, by contrast, branches from glucose-6-phosphate, generating NADPH and ribose-5-phosphate (for nucleotide synthesis) (73)

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