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Within the cytosol of cells, glutathione is kept primarily in the reduced GSH form, largely by reduction of GSSG by the enzyme glutathione reductase, at the expense of NADPH: The mechanism of this reaction is complex, as the enzyme contains a pair of cysteine residues that reversibly form a disulfide bond, plus one FAD molecule (3)

doi: 10.1002/anie.201916316 243 Saporito-MagriC

Solvent A was prepared by mixing 99 mL of Milli-Q (Millipore) water and 1 mL of 1 mol/L ammonium bicarbonate and was adjusted to a pH of 9.2 with 25% ammonium hydroxide (~0.1 mL)

Treatment of symptomatic diabetic polyneuropathy with the antioxidant alpha-lipoic acid: a 7-month multicenter randomized controlled trial (ALADIN III Study)