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The sections were pre-treated with 1% H 2 O 2 in 50% methanol after being deparaffinized, then incubated overnight at 4C with the isolectin B-4 in Tris buffered saline before being developed in DAB

Gahl WA, Bashan N, Tietze F, Bernardini I, Schulman JD (1982) Cystine transport is defective in isolated leukocyte lysosomes from patients with cystinosis