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Plates were washed with PBS-T and anti-mouse IgM-HRP or anti-mouse IgG-HRP (Sigma A8786 and A3673) was used for detection

168 Therefore, inducing ferroptosis may only be effective for certain types of cancer, requiring careful evaluation of cell type- and differentiation state-related signaling pathways that may dictate sensitivity and resistance to ferroptosis

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These include: Genetics Brain injuries, such as from falls or accidents Medical conditions, such as diabetes or high blood pressure Poor diet Lack of exercise Smoking Toxins Medications or improper use of medications Social isolation and loneliness Reduction in sleep Some of the reasons these factors can reduce brain health are that they increase inflammation in the brain and can also cause a change in the hormone and nutrient levels in the brain (1)