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Zhao J, Yang J, Ding L, Wang F, Lin L

coli BL21 DE3 cells expressing NERNST were cultured in LB broth or M9 minimal medium 81 , in the latter case supplemented with 0.2% (w/v) casaminoacids and either 0.5% (w/v) glucose, 1% (v/v) glycerol or 1.2% (w/v) acetate as carbon sources

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The peteridine moiety of the isoalloxazine ring is located in a canonical active site for ubiquinone substrate binding (hereafter named ubiquinone-binding pocket), which is mainly formed by residues 293298 of 10, residues 327331 and 354362 from CTD, residues 340350 from CTD of the other chain in the dimer, where one CoQ 1 molecule is clearly buried in (Figs