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Cell proliferation IMR90 (4 10 4 ) were plated in a 24-well plate and placed onto the stage of the Incucyte Live Content Imaging instrument

doi:10.1155/2020/9210572 Grtner R, Gasnier BC, Dietrich JW, Krebs B, Angstwurm MW

Cell culture, cell treatment, and stable cell line establishment The human renal proximal tubule epithelial cell line HK-2, ccRCC cell lines ACHN, 769-P, 786-O, Caki-1, OSRC-2, and the human umbilical vein endothelial cell line HUVEC were all procured from the American Type Culture Collection (ATCC)

Abnormalities in selenium metabolism and skeletal development diseases Selenium deficiency is regarded as one of the initiating factors of KBD, which is an endemic osteoarthropathy caused by the premature closure of epiphyseal plate and the impaired skeletal development