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Monolayers were washed twice with HBSS and incubated with DMEM D1145 (apical = 0.4 mL and basolateral = 1.5 mL) for 30 min for acclimatization

This comprehensive overview explores the critical blood alcohol concentrations associated with life-threatening outcomes, signs of alcohol poisoning, and the factors influencing the lethal dose, aiming to inform and prevent alcohol-related fatalities