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The appropriate buffer was then used to dissolve SAM from stocks to 500 M

No anesthesia is required

The first step was to determine the best chromatographic conditions for the analysis, the selection of a mobile phase, duration of analysis, the amount of sample to be analyzed, determination of the smallest detectable concentration of the analyte, retention time for GSH and GSSG, limit of detection and quantification, the choice of chromatographic column, and the selection of operating parameters of HPLC instrument (1200 series HPLC system

While physiological concentrations of NO are essential for various cellular functions, its overproduction, particularly from the upregulation of inducible nitric oxide synthase (iNOS), can have deleterious effects in the context of sepsis 54,55