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Acknowledgements The current work was supported by the Chinese National Natural Science Fund (31925013, 32125016, T2321005, U20A20393, 32070907, 31871405, 31870902, W2411011 and U24A20371), a special program of the Ministry of Science and Technology of China (2024YFC2707400, 2021YFA1101000, 2022YFA1105200 and 2023YFA1800200), a Key Research and Development Program of Zhejiang Province (2024C03142), a joint project of the Pinnacle Disciplinary Group, the Second Affiliated Hospital of Chongqing Medical University, the Science Foundation of Jiangsu Province (19KJA550003), the Suzhou Innovation and Entrepreneurship Leading Talent Program (ZXL2022505), a Suzhou Medical College Basic Frontier Innovation cross-research project (YXY2303027), key cross-research projects of the School of Medicine at Soochow University (YXY2303027) and the Suzhou International Joint Laboratory for Diagnosis and Treatment of Brain Diseases

After 1, 5, 10, 15, 20, and 30 min of reduction reaction 5 L of 0.1 mol/L BCPB was added