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Protein preparation The hippocampi were collected and cleaned with PBS

A recent study shows that upregulation of Nrf2 gene expression in a mice experiment led to increased NQO-1 protein content and activity, as well as elevated HO-1 protein content and activity in the brain, while in the liver, HO-1 activity and mRNA levels, NQO-1 activity, and protein content were augmented (92).This study reveals the tissue-specific regulation of Nrf2 signaling and downstream antioxidant enzymes in the mice, highlighting its adaptive response to varying oxygen concentrations

Reduced glutathione (GSH) is the active, usable form