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In mice fed Se deficient diets, testicular atrophy, reduced diameter of the seminiferous tubules, osseous metaplasia, and reduced spermatogenic activity were observed: no mitotic activity in spermatogonia, reduced the number of spermatogenic cell lines (including stem cells, pachytene spermatocytes, spermatids, and maturing sperms), and higher rates of abnormal morphology of sperm were found without difference in chromosomal abnormalities in spermatocytes (85, 100)

Moreover, the analytical validity of LC-MS/MS methodology for quantifying SPMs in clinical samples is another critical aspect to consider, since the current analytical methods cannot accurately detect SPMs levels to the extent, leading that there is controversial about the presence of SPMs in biological matrices

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Cyclosporine A (81 patients, 8 studies) provided an ORR of 63.5% (95% CI, 42-85.1