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After 30 min of blocking with blocking buffer (Dulbeccos PBS containing 0.2% bovine serum albumin, pH 7.4), at RT, the plates were washed 3x with wash buffer (Dulbeccos PBS containing 0.05% Tween 20, pH 7.4)

After separating from the ventral ramus, the nerve travels medially before joining a branch from the ramus gray to contribute sympathetic fibers, then passes through the intervertebral foramen and osteofibrous foramen near the pedicle, reentering the spinal canal

Targeting mitochondrial oxidative stress with MitoQ reduces NET formation and kidney disease in lupus-prone MRL- lpr mice