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doi: 10.1073/pnas.86.14.5296

the cells from tumor and draining lymph nodes tissue were stained with anti-CD11c-PE, anti-CD80-FITC, and anti-CD86-APC antibodies for 30 min, and further analyzed the ratio of mature dendritic cells (DCs)

The whole protein was taken as the search space to include all potential interactions and the conformation with the lowest binding energy was selected