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Growth rates were predicted by setting the biomass reaction in the iCHO2291 model as the objective function and applying enzymatic capacity constraint flux balance analysis (ecFBA), which, unlike conventional FBA (e.g., FBA, FVA, pFBA), integrates enzyme kinetic constraints like turnover numbers ( k cat ) alongside standard FBA constraints, enhancing the accuracy of intracellular flux distributions by accounting for enzyme efficiency and enabling exploration of metabolic resource allocation 33

After full-text assessment, an additional 23 articles were excluded due to irrelevance or insufficient mechanistic detail

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Tanguy S, Morel S, Berthonneche C, Toufektsian M-C, de Lorgeril M, Ducros V, et al