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Description

The tissue sections were stained with Safranin O-Fast Green (SOFG), Hematoxylin & Eosin (H&E) and Sirius Red

Notably, the supplementation of astaxanthin, compared to thermal stress alone, inhibited the expression of inflammatory factors and apoptosis-related genes, including TLR2 , IL8 , EIF4E , IL2RB , CASP3 , and CASP9

To assess this, we evaluated behavioral biomarkers of anxiety-like conditions as well as social behavior, two key behaviors on zebrafish neurotoxicity evaluation