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Description

Science 321 , 563-566 (2008)

Effect of low-level iron and vitamin supplementation on a tropical anemia

Yoon BR, Oh YJ, Kang SW, Lee EB, Lee WW

Briefly, 50 L of pre-cooled extraction buffer 1 [50 mM TrisHCl, pH 7.5, 100 mM NaCl, 1 mM EDTA, 1 mM DTT and 0.5% (w/v) PVPP] was added to 50 mg of roots and ground in liquid nitrogen