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The equilibrated solutions were next concentrated in centrifugal ultrafiltration devices and the eluate containing free L-Asp was collected

Western blotting Cells were lysed with 1% sodium dodecyl sulfate (SDS) buffer containing 50 mM Tris-HCl (pH 7.5), protease inhibitor cocktail (Nacalai Tesque), and PhosSTOP EASYpack (Roche Diagnostics, Mannheim, Germany)

Some products, like soaps and cleansers, are meant to be washed off immediately

Bull Fac Pharm Cairo Univ 51(2):261272 Ahmed TA et al (2022) Development of 3D-printed, liquisolid and directly compressed glimepiride tablets, loaded with black seed oil self-nanoemulsifying drug delivery system: in vitro and in vivo characterization