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The precise regulation of EVs in the physiological processes outlined above underscores their fundamental role in reproductive success

GeneTailor Site-Directed Mutagenesis System (Invitrogen) was used to perform site-directed mutagenesis of the miR-221 binding site in the IGF-1 3'UTR (designated as mut)

Over the next years, it will be important to understand the effect of the reduced circulating amino-acids with the novel multi-agonists targeting the glucagon receptor on lean muscle mass (especially in populations with obesity and high risk of sarcopenia) as well as on energy expenditure [66]