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To fully realize the potential of metabolic/epigenetic modulators, future clinical trials should incorporate analysis of biomarkers to unravel epigenomic (DNA methylation and histone lysine acetylation) and metabolomic (metabolites) markers that allow the selection of subsets of patients that may benefit most from these treatments

The PVDF membrane was subsequently washed (5 min 5) with tris-buffered saline with tween (TBST) buffer and then incubated with the appropriate secondary antibody (Peroxidase-conjugated goat anti-rabbit IgG (H + L), at a dilution of 1:5000) in a 5% bovine serum albumin solution for 1 h at 25 C

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