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To exclude any effects associated with the procedure used for generation of a new monoclonal sub-clone, wild-type HepG2 cells were treated with varying concentrations of NFT or DEM for 24 h, fixed, and subjected to immunocytochemistry to visualize Nrf2 localization (Fig

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Materials and methods Animal We crossed Adiponectin - Cre (C57BL/6 background) mice with Ssu72 flox/flox (Ssu72 WT, C57BL/6 background) mice to generate adipocyte-specific Ssu72 knockout (Ssu72 flox/flox

Sections were mounted with Canada Balsam and imaged using a Leica SP8 confocal microscope