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Validation of the experimental model The RT-qPCR analyses revealed distinctive transcriptional signatures for MOD and SEV forms of COVID-19 and the CTRL group in the aliquots of endpoint Z, which remained mostly preserved after 24h of incubation (endpoint A) ( Figure S2 and Figure 3 )

The main pathways enriched in the inf_CON group were related to triacylglycerol metabolism, acetyl-CoA fermentation to butyrate, and autoinducer 2 (A1-2) transport and processing (lsrACDBFGE) pathways

Research focus: metabolic health, obesity models, and cellular energy dynamics