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Importantly, these findings provide a mechanistic explanation for the limited diagnostic value of circulating zinc measurements and the variable clinical efficacy of nonspecific zinc supplementation, which fail to capture or correct compartment-resolved zinc dysregulation

However, because hepatotoxicity is a result of complex mechanisms, animal studies must be performed in association with such in vitro experiments

A study (322) demonstrated that male Sprague Dawley rats administered low (2.6 g/kg/day), medium (5.3 g/kg/day), or high (10.5 g/kg/day) doses of fructose over 20 weeks exhibited elevated serum levels of pro-inflammatory cytokines (IL-6 and TNF-), alongside a reduction in the anti-inflammatory cytokine IL-10

27 In addition, the uptake, recycling, and clearance of non-heme iron are crucial for regulating irons stable state