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Magnetic beadbased purification is preferred when: Samples are small or low-abundance Fast turnaround is required Minimal equipment is desired High-throughput or automated workflows can be used Traditional methods (e.g., affinity chromatography, ion exchange, filtration) are more suitable for large-scale or preparative protein purification, where higher binding capacity is needed

We discovered that HSA, IgG and the majority of other human plasma proteins are transformed into potent inducers of the neutrophil respiratory burst specifically by N-chlorination [37], the same mechanism by which bacteria were found to employ novel chaperones to protect their proteins against HOCl-stress-induced aggregation [15, 16] (Fig

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