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Description

The unconjugated antibody is transferred into a 50 kDA Amicon Ultra 500 V-bottom filter (Fisher Scientific, Hampton, NH, USA) and reduced for 30 min at 37 C with 1:125 dilution of Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (ThermoFisher, Waltham, MA, USA)

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