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Proteomics reveals specific biological changes induced by the normothermic machine perfusion of donor kidneys with a significant up-regulation of Latexin

Cell lysis was performed by sonication, and lysates were clarified by centrifugation at 17,000 g for 30 min at 4 C

A significant gap in the current understanding is the influence of the gut microbiome on this crosstalk as microbial metabolites could modulate the local availability of both gasotransmitters and their precursors, thereby influencing CRC progression