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In vitro epithelial infection model For our infections, the medium was removed and replaced with prewarmed fresh RPMI-1640 (or RPMI-1640 with L-glutamine but without glucose, catalog number 11879020, Thermo Fisher Scientific) without FBS, and supplemented with or without 10 mg/mL albumin

doi: 10.3389/fmed.2026.1810363 Received 13 February 2026 Revised 18 March 2026 Accepted 23 March 2026 Published 04 May 2026 Volume 13 - 2026 Edited by Ramona Cioboata, University of Medicine and Pharmacy of Craiova, Romania Reviewed by Robert Steven Wallis, The Aurum Institute, South Africa Giuseppe Castaldo, University of Naples Federico II, Italy Updates Copyright 2026 Barne, Shevade, Bhattacharyya, Christopher, Nair, Abhyankar, Halder, Vora, Khanna, Swarnakar, Chhajed, Santhalingam, Nair and Salvi

The roots were then oven-dried at 105 C for 15 min, followed by drying at 75 C until a constant weight was reached

Not only do they help keep your serum fresh and extend its shelf life, but they also make sure the oils stay effective for longer