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Samples were derivatized with o -phthaldialdehyde (OPA) and separated on a C18 column by gradient elution using 0.05 M potassium phosphate buffer (pH 7.0) 3.5 % (vol/vol) tetrahydrofuran mobile phase without or with 40 % (vol/vol) acetonitrile

More crucially, the intestinal microbiota also participates in ferroptosis by facilitating oxidative stress balance and reducing ROS accumulation

For example, fructans can be very supportive of probiotic lactic acid bacteria and stimulate their growth (69), and glucose derived oligosaccharides like polydextrose can increase intestinal Ruminococcus intestinalis and enhance butyrate production (70)