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The generation of mutants for selected genes based on transcriptomic data, using advanced techniques such as CRISPR/Cas9, will further open new horizons for developing resistance to viruses or other multifactorial stresses

[DOI] [PubMed] [Google Scholar] Huang J, Zhang Y, Peng JS, Zhong C, Yi HY, Ow DW, Gong JW

For the orthotopic pancreatic cancer model, VRK2-WT or VRK2-KO Pan02 cells in logarithmic phase (8090% confluence) were harvested, detached with 0.25% trypsin-EDTA, centrifuged (1000 rpm, 5 min), and resuspended in cold serum-free RPMI-1640 medium