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For siRNA transfection experiments, Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) was used according to the manufacturers protocol

As examples, SKOV3 (IC 50 = 0.990.08) and HepG2 (IC 50 =0.870.07) have shown almost the same level of sensitivity, however, after the exposure to cisplatin the GSH level was increased dramatically (about 2.5 times), but decreased significantly (about 2 times) in these cell lines, respectively

Expression and functional role of mGluR3 and mGluR5 in human astrocytes and glioma cells: opposite regulation of glutamate transporter proteins

The functions of these miRNAs are illustrated in Figure 7