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Remarkably, C

Briefly, 30 l of mouse anti-CDC27 antibody (Santa Cruz, sc-9972) coupled to 30 l of protein G-agarose (Roche) was incubated with 2 mL extracts from nocodazole arrested and released G1 (3 h post release) cells, and mixed for 2 h at 4 C

Amide I bands (17001600 cm 1 ) were used to determine the secondary structure of proteins in cell samples from different treatment groups, and spectra of original untreated spectra were synthesized and analyzed using omnic software (Thermo Nicolet, Waltham, United States)

Overall, the 1% CF + 0.13% CA-L and 1% CF + 0.7% CA-L formulations made with Lipoid S 75 had the smallest vesicle sizes, 76.8 nm and 71.4 nm, respectively, followed by the 1% CF-L at 193 nm, and the largest sizes were seen in the CA formulations only