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Huang Y, Liu Z, Liu C, Ju E, Zhang Y, Ren J, Qu X

After additional washing in PBST, nanobodies were diluted four times ten gradients starting at 400 g/mL concentration and were added to the wells and incubated for 2.5 h at 37 C

(B) Western blot of endogenous A39 and ectopically expressed FLAG-tagged A39 WT , substrate binding pocket mutant A39 C334S , and matrix loop 1 four cysteine mutant A39 4CA in the K562 cells upon GSH depletion by BSO treatment (1mM, 2d)