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Methods: Male C57BL/6J mice were fed a high-fat diet with L-NAME for 8 weeks to induce HFpEF, followed by 4 weeks of MET or EMPA treatment

Specifications: Odor and taste: Almost odorless, sour taste Arsenic (As): 2 ppm Heavy Metals : 10 ppm Identification (IR): Corresponds Identification (Lead Acetate): Positive Identification (Ninhydrin): Positive Iron (Fe): 10 ppm Loss on Drying: 0.5 % Optical Rotation: - 21 - -11 Residue on Ignition: 0.1 % Research or further manufacturing use only, not for food or drug use.

In order to gain more insight into the activity of this pathway studies have also looked at the levels of its regulatory proteins, the IGFBP family (Renehan et al., 2004)