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To test this, the PRKAA1 5UTR was inserted upstream of the luciferase CDS (5UTR reporter), as described previously 38 , and transfected into shCtrl and shGDH1 cells for luciferase reporter assays

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Tumor cell-derived HMGB1 has been shown to induce regulatory T cells (Tregs) to produce IL-10, which in turn suppresses CD8 + T cell-dependent antitumor immunity [83]