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Factors such as high pressure and speed, during sorting, dye-induced defects, electrical deviation, changes in pH and osmolarity, etc might lead to changes to the sperm plasma membrane, trigger pre capacitation-like changes, cause protamine decondensation and alter sperm DNA quality (68)

MAST4-sh2 slightly affected the ciliary assembly

Biol Trace Elem Res 76(2):161173

Funding This work was supported by grants from General Program of National Natural Science Foundation of China (82571371), National Key Research and Development Program of China (2021YFC2500102), and Hubei Provincial Clinical Research Center for Dementia and Cognitive Impairment (PTYX2025024)