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Wild type SOD1 can be misfolded by the aberrant translation, post-translational modification or oxidative modifications, due to the pathological conditions of the CNS environment, and produce toxic insoluble aggregates, as mutated SOD1 (Durazo et al., 2009)

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Heart tissues isolated from FLAG-BNIP3 mice were initially washed with PBS, dissected, and homogenised in immunoprecipitation (IP) lysis buffer composed of 1 Cell Lysis Buffer (Cell Signaling), 100 mM PMSF (Roche), and 1 protease and phosphatase inhibitor cocktail (ThermoFisher)