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Description

Subsequent research revealed that CUR increased ALP, COL1, and RUNX2 proteins and mRNA and ALP activity

PMC 3967529

In total, 1 L of cDNA, together with 5 L of SYBR green mix (TIANGEN Biotech, Hainan, China), 0.3 L each forward and reverse primer, were added into the PCR mixtures (with the total volume of 10 L)