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Pelleted bacterial cells were lysed in 30 ml of lysis buffer (20 mM Tris-Cl (pH 7.5), 0.1% Triton X-100, 1 mg/ml lysozyme) for 20 min and sonicated, and insoluble molecules were removed

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The biological role of glutathione and glutathione S-transferases in oxidative stress Numerous studies have shown that in order to avoid prolonged exposure to ROS produced during oxidative stress, the body has a very efficient antioxidant defense system

In contrast to the unchanged levels of PARP1, both the protein levels and enzymatic activity of CD38 are enhanced during aging, contributing to the age-related NAD + decline in mammals