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During both procedures, tail-blood glucose was measured at 0, 15, 30, 45, 60, 90, and 120 minutes with a Contour blood glucose meter (Ascensia Diabetes Care, Parsippany, NJ)

Considering the pharmacokinetics of human growth hormone (hGH), the common practice of administering subcutaneous human growth hormone (hGH) injections in the evening between 20:00 and 21:00 leads to a peak in hormone levels approximately 46 hours later, aligning closely with the natural nocturnal surge that typically occurs around midnight to 02:00 ( Switching to morning GH injections would indeed alter this pattern, causing higher circulating GH levels during daytime hours than under normal physiological conditions

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