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Alternatively, the GST-fusion expression vector can be engineered to encode a recognition site for a specific protease, such as thrombin or factor Xa, between the GST structural gene and the gene of interest

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In this assay, a GFP1-10 fragment was fused to the luminal segment of TMED10 to evaluate the translocation of GFP11-tagged mIL1 into the ERGIC lumen, where GFP complementation occurs