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"I hope the FDA doesn't consider this 'no' vote to mean that we support selling 1,000 acetaminophen tablets in Costco," said Kramer, the Duke University professor

For the IHC study, after deparaffinization (60 C) and antigen retrieval (1 mM citrate, pH 6, 98 C), slides were treated with 3% H 2 O 2 (5 min) and incubated with primary antibodies (1550 min): cleaved caspase-3 (1:300 dilution, Cell Signaling, 9664S), -H2AX (1:400 dilution, Abcam ab22551), HO-1 (1:400 dilution, Abcam ab52947) and CDA (1:300 dilution, Invitrogen PA5-95616) on a Leica Bond RXm autostainer

Nuclei were pelleted by centrifugation (420 g, 4 C for 5 min) and supernatant was discarded