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Furthermore, TNF- interferes with the electron transport chain (ETC), resulting in mtDNA degradation and upregulation of hypoxia-inducible factor-1 alpha (HIF-1), both of which exacerbate hepatic hypoxia and oxidative stress [35, 36]

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To probe this, we tested a small selection of different fluorescent dyes: two that are more membrane permeable, uranine (fluorescein sodium salt) (MW 376.27) and acridine orange (MW 265.35), and one with poor membrane permeability, Texas red (sulforhodamine 101 acid chloride) (MW 625.16)

Since co-treatment of UK-5099 and JMF4073 depleted dTTP pool and suppressed the growth of T315-Bcr-Abl-32D cells, we proposed that reducing GSH biosynthesis induces not only oxidative stress but also replication stress in tumor cells, which demands the synthesis of dTTP and dCTP for survival