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The culture media were replaced every 48 h

A mild deficiency may have no symptoms, but a more severe or prolonged deficiency can exhibit the following: Microcytic anemia Skin conditions Depression Confusion Lowered immunity Certain conditions can increase the risk of developing a deficiency by interfering with the absorption of vitamin B6: Kidney disease Autoimmune intestinal disorders like celiac disease, ulcerative colitis, and Crohns disease Autoimmune inflammatory disorders such as rheumatoid arthritis Alcoholism Toxicity It is quite unlikely to reach a toxic level of vitamin B6 from food sources alone

doi:10.1111/j.1600-0625.2011.01324.x 13

Various inhaled pollutants, whether organic or inorganic, can exert profound effects on neurological health through shared mechanistic pathways, including oxidative stress, systemic inflammation, and BBB disruption