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explains the timing question in more detail

Yang G, Xia Y, Ren W

Professional Use

Polymerase chain reaction (PCR), library preparation, and 16 S rRNA gene sequencing: The V3-V4 hypervariable region of the 16 S rRNA gene was amplified using the primer pair ((Forward = 5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3) and (Reverse = 5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3))