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Once anaesthetised (confirmed by toe-pinch reflex), the mice were intubated endotracheally and ventilated with 3 cm H 2 O positive end-expiratory pressure

This hypothesis was confirmed by several studies that had reported an important correlation between GSH levels and its positive effect on the active vitamin D ratio in the body where they showed that GSH deficiency is associated with increased oxidative stress where they epigenetically alter vitamin D regulatory genes and, as a result, the suppressed gene expression decreases vitamin D biosynthesis, ultimately leading to a secondary deficiency of vitamin D3

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Another in vivo study was carried out by Estrella et al