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3.5.1 Mechanism of action In the brush border of enterocytes lining the intestinal villi, -glucoside inhibitors (AGIs) competitively inhibit -glucosidase enzymes, preventing the enzymes from cleaving disaccharides and oligosaccharides into monosaccharides (167170)

Furthermore, GA inhibited the phosphorylation of PI3K, Akt and ERK, and regulated the expression of inflammatory proteins (reducing the activation of NF-B and Ras, and increasing the expression of iNOS and Kinase Suppressor of Ras 2), and increased the expression of Phosphatase and Tensin Homolog deleted on Chromosome 10 (PTEN), thereby inhibiting TNF--induced cell proliferation (Chung et al., 2020)

The researchers examined participants' blood and muscle tissue at two time points: One week before the cycling test and one day afterwards