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However, existing NADPH sensors face major limitations, including sensitivity to pH, lack of responsiveness to NADP + , signal-to-noise ratio, and compatibility with alternative measurement techniques like FLIM

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Consequently, iron deficiency or impaired metabolism can lead to damage to nigrostriatal neurons and disruption of the dopamine system, which is a primary pathobiological factor in restless legs syndrome (RLS) [73, 74]

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