glutathione protandim parkinson& 39 Targeting the Cysteine Redox Proteome in Parkinson's Disease: The Role of Precursors and Beyond Efficacy of Sulforaphane in Neurodegenerative

The HPLC analysis measures the exact percentage of active target molecules

Membranes were incubated with primary antibodies diluted in 5% milk 1X TBST overnight at 4C: anti-Actin (1:3000) (Sigma, A2066), anti-NRF2 (1:1000) (Cell Signaling Technology #12721) and anti-GSR (1:1000) (ThermoFisher, PA5-29945), followed by washing and incubation with HRP-conjugated anti-rabbit IgG (1:2000) (CST#7074) in 5% milk 1X TBST (22C, 1 h)

Yamaguchi H, Wyckoff J, Condeelis J (2005) Cell migration in tumors

This has been suggested to promote copper deficiency in AD, as astrocyte ApoE4-mediated copper retention could contribute to reduced neuronal copper availability since astrocytes are key to regulating brain copper levels (Scheiber and Dringen, 2013