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5X extraction buffer (50 mM HEPES pH 7.5, 1 M mannitol, 350 mM sucrose, 5 mM EGTA) was added with a final 1X concentration and one stroke was performed with the dounce homogenizer

Front Cell Neurosci 14:282 Kaplan GB, Leite-Morris KA, Wang L, Rumbika KK, Heinrichs SC, Zeng X, Wu L, Arena DT, Teng YD (2018) Pathophysiological bases of comorbidity: traumatic brain injury and post-traumatic stress disorder