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We scrutinize the role of ROS from hepatitis onset through liver fibrosis to HCC growth and migration, discussing their impact on cellular fates such as proliferation, phenotype remodeling, senescence, and cell death

Clarified supernatants were filtered using 0.2-m filters, transferred to new 50-ml Falcon tubes containing Ni-NTA resin equilibrated with lysis buffer (20 mM Tris-HCl (pH 9), 10 mM imidazole, 200 mM NaCl and 5% glycerol) and agitated for 1 h to allow protein binding

By contrast, serine itself is an allosteric inhibitor of PHGDH, and this allosteric inhibition can spontaneously disappear at low serine levels (Sugimoto and Pizer, 1968)